Comparison of the inhibition of monoamine oxidase and butyrylcholinesterase activities by infusions from green tea and some citrus peels.

Ayokunle O Ademosun,Ganiyu Oboh

doi:10.1155/2014/586407

Ayokunle O Ademosun, Ganiyu Oboh

Open Access

https://doi.org/10.1155/2014/586407

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Abstract

This study sought to investigate the effect of infusions from green tea (Camellia sinensis) and some citrus peels [shaddock (Citrus maxima), grapefruit (Citrus paradisi), and orange (Citrus sinensis)] on key enzymes relevant to the management of neurodegenerative conditions [monoamine oxidase (MAO) and butyrylcholinesterase (BChE)]. The total phenol contents and antioxidant activities as typified by their 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals scavenging abilities, ferric reducing antioxidant properties, and Fe2+ chelating abilities were also investigated. Green tea had the highest total phenol (43.3 mg/g) and total flavonoid (16.4 mg/g) contents, when compared to orange [total phenol (19.6 mg/g), total flavonoid (6.5 mg/g)], shaddock [total phenol (16.3 mg/g), total flavonoid (5.2 mg/g)], and grapefruit [total phenol (17.7 mg/g), total flavonoid (5.9 mg/g)]. Orange (EC50 = 1.78 mg/mL) had the highest MAO inhibitory ability, while green tea had the least MAO inhibitory ability (EC50 = 2.56 mg/mL). Similarly, green tea had the least BChE inhibitory ability (EC50 = 5.43 mg/mL) when compared to the citrus peels' infusions. However, green tea infusions had the strongest highest ABTS radical scavenging ability, reducing power, and Fe2+ chelating ability. The inhibition of MAO and BChE activities by the green tea and citrus peels infusions could make them good dietary means for the prevention/management of neurodegenerative conditions.

Highlights

Some of the therapeutic targets that have been identified in the management of neurodegenerative conditions include monoamine oxidase (MAO), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and oxidative stress [1]
The results of the total phenol and flavonoid contents of the infusions from green tea and the citrus peels as presented in Table 1 revealed that green tea had the highest total phenol (43.3 mg/g) and total flavonoid (16.4 mg/g) content, Fe2+ chelating ability (%)
The BChE inhibitory abilities of the infusions revealed that green tea had the least inhibitory ability (EC50 = 5.43 mg/mL) when compared to the other infusions [orange (EC50 = 3.61 mg/mL); shaddock (EC50 = 4.42 mg/mL); grapefruit (EC50 = 4.20 mg/mL)]

Summary

Introduction

Some of the therapeutic targets that have been identified in the management of neurodegenerative conditions include monoamine oxidase (MAO), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and oxidative stress [1]. The use of MAO inhibitors and their mechanism of action in the management of depression and other neurodegenerative conditions such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) have been established [2]. Inhibiting MAO activity is a therapeutic target in the management of AD and other neurodegenerative conditions. The use of AChE and BChE inhibitors to reduce the hydrolysis of acetylcholine is a therapeutic approach in managing neurodegeneration. Efforts aimed at ameliorating oxidative damage have been shown to be beneficial in the treatment of neurodegenerative conditions. Green tea and citrus peels have been in use in alternative and complementary medicine as a cheap intervention in the management of some degenerative

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