Comparison of the Influence of “Solvent/Non-Solvent” Treatment for the Attachment of Signal Molecules on the Structure of Electrospun PCL and PLLA Biodegradable Scaffolds

Semen Goreninskii,Evgeny Bolbasov,Nadezhda Danilenko,Victor Filimonov,Ksenia Stankevich,Sergei Tverdokhlebov

doi:10.1051/matecconf/20167901025

Semen Goreninskii, Evgeny Bolbasov + Show 4 more

Open Access

https://doi.org/10.1051/matecconf/20167901025

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Abstract

Electrospun biodegradable scaffolds (matrixes) made of poly(e-caprolactone) (PCL) and poly(L-lactic acid) (PLLA) are three-dimensional fibrous structures that are commonly used in regenerative medicine and drug delivery systems. Modification of such structures allows manipulating with biological and immune response. Previously, our team suggested a number of surface modification strategies for thin films made of PLLA. One of the proposed strategies are based on treatment of the material with “solvent/non-solvent” mixture that allows absorbing biologically active molecules or linkers on the surface of the sample. The aim of this work was to compare the influence of “solvent/non-solvent” treatment on the structure and crystallinity of the elecrospun biodegradable PCL and PLLA scaffolds. For that purpose, original PCL and PLLA scaffolds were treated with mixture of toluene and ethanol in different proportions. Morphology of the obtained samples was studied using scanning electron microscopy. It was shown that “solvent/non-solvent” treatment doesn’t lead to changes in scaffolds morphology such as gluing or cutting of the matrix fibers. By means of X-ray diffraction analysis it was shown that treatment of the samples with selected mixtures doesn’t change material crystallinity. Thus, it was demonstrated that proposed composition of the “solvent/nonsolvent” mixture can be used for the modification of electrospun PCL and PLLA scaffolds.

Highlights

Poly(L-lactic) acid (PLLA) and poly(ε-caprolactone) are biodegradable polymers widely applied in modern medicine [1]
3.1 Scaffolds morphology scanning electron microscopy (SEM) images of the original and treated PCL and PLLA scaffolds are shown in the Figure 2
It was found that produced PCL scaffolds have bimodal distribution of the fiber diameter, whereas PLLA scaffolds demonstrate unimodal distribution

Summary

Introduction

Poly(L-lactic) acid (PLLA) and poly(ε-caprolactone) are biodegradable polymers widely applied in modern medicine [1] Because of their outstanding properties, such as degradability [2], processability [3] and biocompatibility [4] these materials are used in production of the wide range of biomedical devices such as catheters [5], coronary stents [6], and implants [7]. Solution blow spinning (SBS) [10] and electrospinning (ES) [11] are common techniques allow for the production of free-dimensional fibrous structures called matrixes (scaffolds) Because of their specific architecture, biodegradable scaffolds are being used in tissue engineering as an extracellular matrix for support of the cell growth [12]. Their biodegradability and controllable degradation rate give an opportunity for the target drug delivery [13]

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