Comparison of the in vitro anti-inflammatory effect of Sanhuang decoction and its composition of traditional Chinese medicne

Abstract

Objective To compare the treatment effect of Sanhuang decoction and its composition of traditional Chinese medicne(Rhubarb, Scutellaria, Phellodendron) on RAW264.7 cell inflammation model, thus to determine whether the effect of Sanhuang decoction on soft tissue injury is stronger than the composition of traditional Chinese medicne used alone. Methods The clean Sprague-Dawley(SD) mice were selected and randomly divided into two groups according to the digital table.RAW264.7 cells were induced inflammation by LPS.The cell viability was tested by MTT method, and the content of the inflammatory factors(PGE2, IL-1beta, NO, TNF-alpha) were detected. Results Compared with the blank control group, the content of PGE2 in the model group increased significantly[(224.95±20.26)pg/mL vs.(72.86±5.57)pg/mL, t=17.769, P<0.01]. Compared with the model group, Sanhuang high, medium and low dose group could significantly inhibit RAW264.7 cells to release PGE2(F=12.141, P<0.01). Rhubarb medium and low dose group, Scutellaria high dose group, Phellodendron high, medium and low dose group could inhibit RAW264.7 cells to release PGE2 (F=12.154, P<0.05). Compared with the blank control group, the content of IL-1 beta in the model control group increased significantly[(26.68±2.61)pg/mL vs.(16.78±0.74)pg/mL, t=11.254, P<0.01]. Compared with the model group, Sanhuang high, medium and low dose group significantly inhibited RAW264.7 cells to release IL-1beta(F=11.547, P<0.01). Rhubarb high dose group, Scutellaria high, medium dose group inhibited RAW264.7 cells to release IL-1 beta(F=11.563, P<0.05). Conclusion The in vitro anti-inflammatory effect of Sanhuang decoction is better than its composition of traditional Chinese medicne(Rhubarb, Scutellaria, Phellodendron) alone. Key words: Drugs, Chinese herbal; Anti-inflammatory agents; Comparative effectiveness research