Abstract
Toxoplasmosis is a disease which can cause severe congenital infection and is normally diagnosed by the detection of T. gondii specific antibodies in the serum of infected patients. Several different tests allow to distinguish recent from past infections and to quantify anti-T. gondii specific IgG, and the results can be used as markers for immunity. In the present study, we compare the performance of two different methodologies, the Elfa (bioMerieux S.A) and the Meia (Abbott Laboratories) in detecting T. gondii specific IgG in low-reactivity sera. Of 76 analyzed samples, three presented discrepant results, being positive in the Abbott AxSYM Toxo IgG assay, and negative in the bioMerieux Vidas Toxo IgG II assay. By using other tests, the three sera were confirmed to be negative. The results are discussed in the context of their importance for patient management, especially during pregnancy.
Highlights
Toxoplasmosis caused by Toxoplasma gondii is a disease distributed worldwide, affecting almost 60%of the European population, 70% of the people living in the Americas, and 75% of the African and Asian populations.pathology associated with pathologies of the fetus and premature abortions
We selected 76 serum samples that were weakly reactive in a semi-automated assay for T. gondiispecific IgG, the AxSYM Toxo IgG assay
The RI values show that the reactivity was higher in the Vidas Toxo IgG II assay, when compared to the AxSYM Toxo IgG assay
Summary
Toxoplasmosis caused by Toxoplasma gondii is a disease distributed worldwide, affecting almost 60%of the European population, 70% of the people living in the Americas, and 75% of the African and Asian populations.pathology associated with pathologies of the fetus and premature abortions. Toxoplasmosis caused by Toxoplasma gondii is a disease distributed worldwide, affecting almost 60%. Of the European population, 70% of the people living in the Americas, and 75% of the African and Asian populations. Pathology associated with pathologies of the fetus and premature abortions. It is necessary to investigate the use of safe diagnostic methods that allow distinguishing recent from past infections, and seropositive from seronegative individuals. Apart from its form acquired by contact with cysts from the environment, toxoplasmosis is the main. Laboratory diagnosis was performed using the traditional serologic profile of toxoplasmosis, 1. 2. Institute for Tropical Medicine, São Paulo, Brazil. Comparison of semi-automatized assays for anti-T. gondii IgG detection in low-reactivity serum samples
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