Comparison of Schmallenberg virus antibody levels detected in milk and serum from individual cows

Janet M Daly,Rachael A Tarlinton,Roger Blowey,Ben C Maddison,Kevin C Gough,Barnabas King

doi:10.1186/s12917-015-0365-1

Janet M Daly, Rachael A Tarlinton + Show 4 more

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https://doi.org/10.1186/s12917-015-0365-1

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Abstract

BackgroundSchmallenberg virus (SBV) is a recently emerged virus of ruminants in Europe. Enzyme-linked immunosorbent assays (ELISA) are commonly used to detect SBV-specific antibodies in bulk tank milk samples to monitor herd exposure to infection. However, it has previously been shown that a bulk tank milk sample can test positive even though the majority of cows within the herd are seronegative for SBV antibodies. Development of a pen-side test to detect antibodies in individual milk samples would potentially provide a cheaper test (for which samples are obtained non-invasively) than testing individual serum samples by ELISA. Therefore, the aim of this study was to investigate the agreement between antibody levels measured in milk and serum.ResultsCorresponding milk and serum samples from 88 cows in two dairy herds in the UK were tested for presence of immunoglobulin G antibodies to SBV using a commercially-available indirect ELISA. A serum neutralisation test (NT) was also performed as a gold standard assay. The ELISA values obtained for the bulk tank milk samples corresponded with the mean values for individual milk samples from each herd (bulk tank milk values were 58% and 73% and mean individual milk values 50% and 63% for herds A and B, respectively). Of the 88 serum samples tested in the NT, 82 (93%) were positive. Although at higher antibody levels, the ELISA values tended to be higher for the individual milk samples than for the corresponding serum samples, the positive predictive value for milk samples was 98% and for serum samples 94%. The serum ELISA was more likely to give false positive results around the lower cut-off value of the assay.ConclusionsThe results indicate that testing of individual milk samples for antibodies against SBV by ELISA could be used to inform decisions in the management of dairy herds such as which, if any, animals to vaccinate.

Highlights

Schmallenberg virus (SBV) is a recently emerged virus of ruminants in Europe
The Enzyme-linked immunosorbent assays (ELISA) is only semi-quantitative, the mean of the individual milk sample values was consistent with the bulk tank milk sample values
A significantly lower mean antibody level was obtained for individual milk samples from herd A (50%) than for herd B (63%)

Summary

Introduction

Schmallenberg virus (SBV) is a recently emerged virus of ruminants in Europe. Enzyme-linked immunosorbent assays (ELISA) are commonly used to detect SBV-specific antibodies in bulk tank milk samples to monitor herd exposure to infection. It has previously been shown that a bulk tank milk sample can test positive even though the majority of cows within the herd are seronegative for SBV antibodies. Schmallenberg virus (SBV), which emerged recently in Europe, causes subclinical or mild disease in adult ruminants with clinical signs including diarrhoea, fever and drop in milk yield in dairy cattle. The first indirect enzyme-linked immunosorbent assay (ELISA) to detect SBV-specific antibodies in serum or milk samples became commercially available shortly after the emergence of SBV [2]. SBV has spread rapidly across Europe and high levels of seroprevalence in cattle have been reported (reviewed in [4]). In addition to regional variation in seroprevalence, higher rates have been reported for herds that graze outdoors compared to herds that are housed

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