Comparison of reversed-phase liquid chromatography columns using “rohrschneider” type constants

Abstract

Using a set of aromatic reference compounds, the retention characteristics of reversed-phase high-performance liquid chromatographic columns can be expressed in terms of “Rohrschneider” type constants. These are based on the differences in retention indices of the reference compounds using the alkylarylketone scale, between the test column and solvent conditions and an ideal hexane—water partition system. The system can be used to quantitatively differentiate between phases coated with different alkyl chains and phenyl groups, and could be used to define the retention specifications of a column.