Abstract
The separation of benzo[ a]pyrene diolepoxide-nuicleoside and deoxynucleoside adducts on μ-Bondapak C 18, Radial-Pak C 18, Partisil ODS-2, Micropak MCH-5 Zorbax ODS and Ultrasphere ODS high-performance liquid chromatographic columns has been compared under identical solvent conditions. The nucleoside and deoxy-nucleoside adducts were obtained by chemical and enzymatic digestion of the nucleic acid products of the reactions of benzo[ a] pyrene diolepoxides with polyguanylic acid and DNA. Significant differences in separation were observed with the columns tested which could not be correlated with particle size, carbon content, surface area, or surface coverage. Baseline separation of eight diolepoxide-guanosine adducts was obtained on the Ultraphere ODS column. The separation of the diolepoxide- deoxynucleoside adducts on the Ultrasphere ODS column suggests that it will be useful in identifying new benzo[ a]pyrene DNA adducts.
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