Abstract
Comparison of Real-time PCR versus ELISA in the diagnosis of cytomegalovirus infection in pregnant women
Highlights
IntroductionThe polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific deoxyribonucleic acid fragments is induced from very low concentrations of complex substrates allowing the detection of very low amounts of viral particles
This study demonstrated that the real time polymerase chain reaction test is more accurate than serological enzyme linked immunosorbent assay (ELISA) test in the diagnosis of cytomegalovirus infection among pregnant women
Diagnosis of CMV disease is based on clinical symptoms, but the symptoms of CMV can be confused with those due to Epstein-Barr virus (EBV), and this may lead to difficulties in diagnosis
Summary
The polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific deoxyribonucleic acid fragments is induced from very low concentrations of complex substrates allowing the detection of very low amounts of viral particles. The most frequently serological used method for detecting immunoglobulin M and immunoglobulin G antibodies are the enzyme linked immunosorbent assay (ELISA). The polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific DNA fragments is induced from very low concentrations of complex substrates [4]. The high sensitivity of the polymerase chain reaction allows the detection of very low amounts of viral particles (DNA or RNA) and several studies have reported the utility of this technique for the quantification of CMV DNA in blood or urine [5,6]
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