Abstract
Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is possibly the most important disease of Brassica worldwide. To compare chromosomal positions of Xcc resistance loci in Brassica oleracea between the present and published studies and to develop marker assisted selection (MAS) to resistance against Xcc race 1, we constructed a B. oleracea map, including pW, pX and BoCL markers that were closely linked to previously reported Xcc resistance QTLs. We also analyzed Xcc resistance QTLs by improving our previously reported map derived from the cross of a susceptible double-haploid line (GC P09) with a resistant double-haploid line (Reiho P01). In the nine linkage groups obtained (C1-C9), the major QTL, XccBo(Reiho)2, was derived from Reiho with a maximum LOD score (7.7) in C8. The QTL (LOD 4.4) located in C9, XccBo(GC)1 was derived from the susceptible GC. The other QTL (LOD 4.4), XccBo(Reiho)1, was found in C5. Based on common markers, it was possible to compare our finding Xcc resistance QTLs with the B. oleraceaXcc loci reported by previous authors; XccBo(Reiho)1 and XccBo(GC)1 may be identical to the Xcc resistance QTLs reported previously or a different member contained in the same resistance gene cluster. Our map includes public SSR markers linked to Xcc resistance genes that will promote pyramiding Xcc resistance genes in B. oleracea. The present study will also contribute to a better understanding of genetic control of Xcc resistance.
Highlights
Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (Pam.) Dowson (Xcc), is the most destructive disease in crucifer crops [1]
It was possible to compare our finding Xcc resistance QTLs with the B. oleraceaXcc loci reported by previous authors; XccBo(Reiho)1 and XccBo(GC)1 may be identical to the Xcc resistance QTLs reported previously or a different member contained in the same resistance gene cluster
The V-shaped lesion was regarded as a triangle shape to calculate disease leaf area (DLA)
Summary
Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (Pam.) Dowson (Xcc), is the most destructive disease in crucifer crops [1]. Xcc enters leaves through insect, or mechanically wounded tissue [2]and through hydathodes at leaf margins and spreads through vascular tissue, clogging vessels and producing V-shaped chlorotic lesions [3]. Such symptoms lead to a systemic infection in susceptible plants so that crop quality and yield substantially decrease. The pathogen can be retained in seeds via vessels and causes severe incidence in descent seedlings; Xcc is difficult to prevent by agricultural practices such as seed treatment, crop rotation and use of agrochemicals. Utilization of Xcc resistant cultivars is one of the most effective approaches to minimize crop loss from infection of the pathogen
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