Abstract
Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), produces V-shaped chlorotic lesions on the leaves of cabbage (Brassica oleracea var. capitata L.), causing darkened veins and drastically reducing yield and quality. Of the 11 Xcc races identified, races 1, 4, and 6 are predominant globally. In the present study, we aimed to develop a molecular marker linked to black rot resistance against Xcc races 6 and 7. Crossed between black rot-resistant (‘SCNU-C-3470’) and -susceptible (‘SCNU-C-3328’) lines obtained 186 F2 plants. Resistance to Xcc race 6 segregated in a 3:1 (susceptible:resistant) ratio in the F2 population, which is consistent with a monogenic recessive trait. Nucleotide-binding site (NBS) leucine rich repeat (LRR)-encoding resistance (R) genes play a crucial role in plant defenses to various pathogens. The candidate R gene (Bol031422) located on chromosome C08, previously reported by our research group, was cloned and sequenced in resistant and susceptible cabbage lines. The R gene Bol031422 consisted of a single exon with a 3 bp insertion/deletions (InDels), a 292 bp polymorphism (an insertion in the exon of the resistant line relative to the susceptible line) and several single nucleotide polymorphisms (SNPs). Here, we developed the InDel marker BR6-InDel to assess linkage between variation at Bol031422 and resistance to Xcc races 6 and 7. This marker will help cabbage breeders develop cabbage cultivars resistant to Xcc races 6 and 7.
Highlights
Cabbage (Brassica oleracea var. capitata L.) is an important vegetable in Brassica crops worldwide due to its high nutritional quality, good storage properties, and potential health benefits [1,2]
We assessed the inheritance patterns of resistance to Xanthomonas campestris pv. campestris (Xcc) races 6 and 7 using an F2 population derived from a cross between the two cabbage lines ‘SCNU-C-3328’ and ‘SCNU-C-3470’, which differ in their resistance to black rot
A χ2 test revealed that resistance to Xcc race 6 follows a 3:1 segregation ratio, which is consistent with a single recessive gene conferring resistance (Table 1 and Figure S1)
Summary
Cabbage (Brassica oleracea var. capitata L.) is an important vegetable in Brassica crops worldwide due to its high nutritional quality, good storage properties, and potential health benefits [1,2]. Capitata L.) is an important vegetable in Brassica crops worldwide due to its high nutritional quality, good storage properties, and potential health benefits [1,2]. Black rot is the most devastating disease of Brassica vegetables, caused by the aerobic, Gram-negative, and nonsporulating bacterium Xanthomonas campestris pv. Black rot substantially reduces the yield and quality of cabbage harvests. 11 pathogenic Xcc races have been identified that can infect Brassica crops [7,8,9]. Races 1, 4, and 6 are the most widespread worldwide and the most aggressive against B. oleracea crops [10]
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