Abstract
We investigated the behavior of Peribacillus simplex in ESL milk by assessing its heat resistance and low-temperature growth. The D-values at 90 °C of P. simplex strains were 3.23 ± 0.09 to 32.23 ± 5.71 min, significantly higher than those of Paenibacillus odorifer but not significantly different those of Bacillus cereus. The z-values of P. simplex (7.49 ± 0.06 to 9.44 ± 0.60 °C) did not differ significantly from those of the other two organisms. The heat-resistance experiments showed that P. simplex would survive process conditions of 120 °C for 2 s. P. simplex grew more slowly than P. odorifer at 4 °C, faster than both comparison species at 7 °C, and as fast as B. cereus at 10 °C. Therefore, depending on the number of spores remaining and the distribution temperature, P. simplex may be the predominant species in ESL milk.
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