Abstract
women. This study aimed to screen the antimicrobial susceptibility and the virulent factors of SA strains isolated from patients with puerperal mastitis. Methods: All SA isolated from the clinical specimens obtained from patients with puerperal mastitis were tested. Susceptibility to common antimicrobial agents was determined using the disk diffusion method. The genes encoding antimicrobial resistance(mecA) and virulent factors encoding the exotoxins(pvl, eta, etb, and tsst) and classic staphylococcal enterotoxins (SEs)(sea, seb, sec, sed, and see) were tested by specific PCR. Some strains were selected for multilocus sequence typing(MLST). Results: A total of 76 SA isolates were obtained from 48 women, 51(67.1%) isolates were oxacillin resistant(ORSA). Most ORSAs were susceptible to the antimicrobial agents [Susceptible rates: gentamicin(67.3%), TMP/ SMX(98.0%), 100% to teicoplanin, rifampin, fusidic acid, linezolid, levofloxacin, doxycycline], but resistant to clindamycin(6.1%) and erythromycin(6.1%); while most of the OSSA isolates were susceptible to clindamycin(74.1%) and erythromycin(74.1%). All but one ORSA carried mecA gene. Of the 76 SA strains, 42.1%(32/76) were positive for pvl, 68.4%(52/76) were positive for at least one enterotoxin genes. Few isolates carried eta 8/76(10.5%), etb 0/76, and tsst 3/76(3.9%). Sequence type 59(ST 59) was the most common(26 strains) type among the 29 SA with MLST performed. Conclusions: Oxacillin resistance was common among SA isolated from puerperal mastitis, but some other common antimicrobial agents remained susceptible, e.g., fusidic acid and TMP/SMX. The association with abscess formation and pvl positive SA infection is not significant.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.