Abstract
Four serological assays for detection of antibody reactivity against surface antigens of benign and malignant cells have been evaluated. In all four assays, antibody attached to the surface of a target cell is detected by a rosette of indicator red blood cells. Of the four assays (antibody mixed hemadsorption assay, immune adherence assay, anti-C3 mixed hemadsorption assay and protein A assay), the anti-C3 mixed hemadsorption assay showed highest sensitivity. By using purified IgM and IgG fractions we could demonstrate that, except for the protein A assay, all assays show reactivity both with IgG and IgM. Protein A assay detected only the IgG fraction, with high sensitivity. The optimization of the four assays is described and the importance of using multiple assays is emphasized.
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