Abstract
Salmonella surveillance and outbreak management is a key function of public health. Laboratories are shifting from antigenic serotype determination to molecular methods including microarray or whole genome sequencing technologies. The objective of this study was to compare the Check&Trace Salmonella™ DNA microarray (CTS), a commercially available assay with the Salmonella in silico typing resource (SISTR), which uses whole genome sequencing technology for serotyping clinical Salmonella strains in Alberta, Canada, collected over an 18-month period. A high proportion of isolates (96.3%) were successfully typed by both systems. SISTR is a powerful tool for laboratories which already have a WGS infrastructure in place, whereas smaller laboratories can benefit from a commercial microarray system and reduce the processing cost per isolate compared to traditional serotyping.
Highlights
Salmonella is a foodborne pathogen and a major source of gastrointestinal infection, with an estimated 93.8 million cases per year [1]
The Alberta Precision Laboratories—Public Health Laboratory (ProvLab) in Alberta is a PulseNet Canada member that plays an important role in supporting PulseNet surveillance initiatives and outbreak management by serotyping human clinical Salmonella isolates submitted by laboratories across the province
Salmonella serotyping was performed using the White–Kauffman–Le Minor (WKL) scheme, which is based on immunological reactions to somatic (O) and flagellar (H) antigens [2]
Summary
Salmonella is a foodborne pathogen and a major source of gastrointestinal infection, with an estimated 93.8 million cases per year [1]. Public health surveillance reduces the public health burden of salmonellosis by rapidly detecting outbreaks, identifying sources, limiting transmission, and preventing future occurrences. In Canada, PulseNet is a vital surveillance system for pathogens associated with foodborne disease. The Alberta Precision Laboratories—Public Health Laboratory (ProvLab) in Alberta is a PulseNet Canada member that plays an important role in supporting PulseNet surveillance initiatives and outbreak management by serotyping human clinical Salmonella isolates submitted by laboratories across the province. Serotyping is crucial for the rapid and accurate detection of Salmonella outbreaks and is integral to the resolution process. Salmonella serotyping was performed using the White–Kauffman–Le Minor (WKL) scheme, which is based on immunological reactions to somatic (O) and flagellar (H) antigens [2]
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