Abstract
Various studies have been published suggesting modifications of technique for the avoidance of the cloudy reaction in conducting agglutination tests for carriers of Bacillary White Diarrhea. It therefore seemed desirable to undertake comparative tests of the modified antigens suggested to determine which would most regularly give clear cut positive or negative agglutination reactions without the interference of nonspecific precipitations. This paper gives the results of these studies.According to Hektoen1 some of the earliest reported observations regarding non-specific precipitations when fowl serum is used in sereologic tests were made by Sutherland and Mitra in 1914. These men were using fowl serum in the precipitin reaction and arrived at the opinion that avoiding freezing of the serum would serve as a means of preventing such non-specific precipitations.Hektoen (1918) doing similar work found the freezing of fowl serum was not wholly responsible for the non-specific precipitation, but he also suggests avoidance .
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