Abstract
Objective: Tuberculosis (TB) one of the most important major causes of mortality and morbidity around the world. Early detection of TB affords to prevent transmission of TB and better treatment options. The aim of this study is to evaluate sensitivity of fluorescent, Ziehl Neelsen microscopy and Polymerase Chain Reaction (PCR) method. Methods: Fifty samples of clinically suspected cases of pulmonary tuberculosis were collected and processed for Mycobacteria by Fluorescent and ZN staining. Negative samples by staining methods were processed by PCR method using primer IS6110 of M. tuberculosis complex. Results: In total, 33/50 (66.0%) samples were positive by Fluorescent and 23/50 (46%) were positive by ZN method. All microscopically negative samples (n=17) were processed to PCR and only seven of samples (41.1%) showed positive results. Conclusion: We concluded that PCR is best but it required lot of investment. J Microbiol Infect Dis 2014; 4(4): 141-144
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
