Comparison of micro-CT and CBCT in the preparation of teeth for the role of samples in in vitro research

Abstract

Introduction. The thickness of dentine is an important factor affecting the results of experimental studies. An adequately precise and repeatable method for its measurement is of key importance for ensuring the reliability and credibility of research. Aim. The aim of this study was to compare conical beam computed tomography (CBCT) and microtomography (micro-CT) for the preparation of teeth as specimens in medical research. Material and methods. From among 100 extracted teeth, 4 teeth with appropriate structural features confirmed in CBCT images were selected. They were embedded in silicone mass and cut in a horizontal plane in such a way that the intersection plane was parallel to the plane defined by the pulp horns. Next, the samples were subjected to conical beam computed tomography and microtomography. Two authors independently measured the distance between the plane of intersection and the pulp horns on the obtained frontal and sagittal sections. The obtained measurements were statistically processed. Results. The values obtained with CBCT were higher than those obtained with micro-CT. Statistically significant differences were found for two of four samples. Conclusions. CBCT is not sufficient for precise preparation of teeth for the role of research specimens.