Abstract
BackgroundThe mouse model of arterialized orthotopic liver transplantation (AOLT) has played an important role in biomedical research. The available methods of sutured anastomosis for reconstruction of the hepatic artery are complicated, resulting in a high incidence of complications and failure. Therefore, we developed and evaluated a new model of AOLT in mice.Materials and methodsMale inbred C57BL/6 mice were used in this study. A continuous suture approach was applied to connect the suprahepatic inferior vena cava (SHVC). The portal vein and infrahepatic inferior vena cava (IHVC) were connected according to the "two-cuff" method. The common bile duct was connected by a biliary stent. We used the stent (G3 group) or aortic trunk (G2 group) to reconstruct the hepatic artery. The patency of the hepatic artery was verified by transecting the artery near the graft after one week. The survival rate of the recipients and serum alanine aminotransferase (ALT) levels, hepatic pathologic alterations, apoptosis and necrosis were observed at one week postoperatively.ResultsThe patency of the hepatic artery was verified in eight of ten mice in G3 and in six of ten mice in G2. The 7-day survival rate, extents of necrosis and apoptosis, and TGF-β levels were not significantly different among the three groups (P>0.05). However, the serum ALT levels and operation time were markedly lower in G3 compared with G2 or G1 (both P<0.05).ConclusionsReconstruction of the hepatic artery using a stent can be performed quickly with a high rate of patency. This model simplifies hepatic artery anastomosis and should be promoted in the field of biomedical research.
Highlights
Hepatocellular carcinoma (HCC) is the sixth most common carcinoma worldwide and is one of the leading causes of cancer-related death [1]
The serum alanine transaminase (ALT) levels and operation time were markedly lower in G3 compared with G2 or group was 12.0 min (G1)
The reconstruction of the hepatic artery by stent can save approximately 4.6 min compared with the aortic trunk method
Summary
Hepatocellular carcinoma (HCC) is the sixth most common carcinoma worldwide and is one of the leading causes of cancer-related death [1]. In 1973, Lee et al introduced for the first time a rat model of OLT [3] This model involved vascular reconstruction by the suture technique, which is extremely complicated and causes anhepatic phase extension. The end-to-side suture anastomosis for reconstructing the hepatic artery has been the classical technique in the mouse model of AOLT. Because the procedure is extremely complex, it requires additional time, and the longer arterial segment is more inclined to kink and lead to a subsequent thrombosis These characteristics result in a high incidence of complications and failure. MicroRenathane implantation tubing is costly, and the difference between reconstruction of the hepatic artery by stent compared with end-to-side suture anastomosis for hepatic artery reconstruction by the aortic trunk remains unknown. The mouse model of arterialized orthotopic liver transplantation (AOLT) has played an important role in biomedical research. We developed and evaluated a new model of AOLT in mice.
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