Comparison of methods for the determination of β-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents

Abstract

β-Aspartylglycine is an indicator of the absence of bacterial enzymatic activity in the intestinal tract. This study describes and compares four methods — (1) dansylation with thin-layer chromatography, (2) ion-exchange chromatography, (3) thin-layer electrophoresis, (4) high-voltage paper electrophoresis — to determine the concentration of β-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents.