Comparison of May Grunwald Giemsa and Potassium Hydroxide Examination of Malassezia Folliculitis Patient Dermatologic Outpatient Clinic of Dr. Soetomo General Hospital Surabaya

Abstract

Background: Malassezia folliculitis (MF) is a pilosebaceous follicle infection caused by Malassezia sp ., in the form of papules, solid, diameter <1cm, follicular pustules, moderate itchy, in the upper body, and rarely on the face. Microscopic examination with potassium hydroxide (KOH) found spores. May Grunwald Giemsa (MGG ) cytologic staining to diagnose MF spores may demonstrate a more detailed and sharper. Objective: Identification of MF with MGG compared KOH in Dermatology outpatient clinic Dr. Soetomo General Hospital. Methods: The study was observational analytic to identify MF using MGG compared KOH. Involving 52 new patients fulfield the criteria of the study from March to May 2016. Results: An examination of 52 patients with MF, 7 patients (13.5%) were negative with KOH examination, while the examination MGG negative in 2 patients (3.8%), but not statistically significant (p ≥ 0.5 ). PPV=90% ; NPV value=100% ; sensitivity=95,7% ; spesificity=28,5% Conclusion: KOH still a good investigation for diagnose of MF, but if clinically result positive MF whereas negative KOH, MGG examination should be performed as a diagnostic confirmation of MF