Abstract
Objectives Bioavailability of NO can be estimated by measuring the concentration of nitrate (NO 3) in serum. However, the methods used for the measurement NO 3 in plasma or serum show a great degree of variation. Therefore, we compared two analytical methods for the measurement of NO 3 in serum. Design and methods The concentration of NO 3 in 600 serum samples collected from healthy individuals was determined by the HPLC and by the Griess reagent-spectroscopic method. Results The concentration of NO 3 in the samples was 29.4 ± 16.1 μmol/L and 26.2 ± 14.0 μmol/L (mean ± SD) measured by HPLC and Griess reagent-spectroscopic method respectively ( p < 0.0001). We detected a significant correlation between the two methods ( R = 0.81, p < 0.0001). Conclusions A significant correlation between the two methods may suggest that either method can be used for the measurement of NO 3 in serum, however the Griess reagent-spectroscopic method measures lower concentrations of NO 3 than the HPLC method.
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