Abstract
ABSTRACTThe results of reverse phase high performance liquid chromatographic (HPLC), competitive binding radiometric, and Lactobacillus casei methods were compared for the determination of total folacin in raw cabbage, a fortified cereal, and a fortified infant formula. A cation exchange procedure was developed which permitted the analysis of cabbage folacin by reverse phase HPLC after hydrolysis of folacin polyglutamates with endogenous conjugase. Although general agreement was observed between assays for the cereal product, marked variation was found for cabbage and the infant formula. These results suggest that the accuracy of the radiometric and L. casei assays may be influenced by the distribution of folacin derivatives present and other sample components. Reverse phase HPLC provides a direct analysis which is not subject to such variability.
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