Abstract
We studied gene expression of five metallothioneins (MT 1-5), ubiquitin and protein p53 and their products in fibroblasts culture of the skin and lungs of white rats of different ages (2 weeks, 1, 3, and 24 months) and determined its (metallothionein 1-5 types, ubiquitin, p53) product quantity. All these proteins are protective ones, but perform their functions by using different mechanisms. Metallothionein bind, transport and excrete ions of bivalent metals, ubiquitin controls the cleavage of the defective and short-lived proteins in the proteasome, protein p53 controls apoptosis, thus ensuring the genome stability. The similarity of age dynamics of gene expression of ubiquitin and MT of cells of both sources has been shown – maximum at 3 months. Expression of p53 gene has a difference: both in the skin and lungs expression increases up to 24 months. Product quantity of p53 has a minimum in the skin at 3 months and remains constant; in the lungs, this value has a maximum at 1 month.
Highlights
Fibroblasts in cell culture from lungs and skin of white rats of different age (2 weeks, 1, 3, and 24 months) have been studied
We studied gene expression of five metallothioneins (MT 1-5), ubiquitin and protein p53 and their products in fibroblasts culture of the skin and lungs of white rats of different ages (2 weeks, 1, 3, and 24 months) and determined its product quantity
Numerous works in the field of developmental biochemistry are still held mainly at the organ and tissue level without the use of experiments with cell cultures (Muller, 2007)
Summary
Fibroblasts in cell culture from lungs and skin of white rats of different age (2 weeks, 1, 3, and 24 months) have been studied. Numerous works in the field of developmental biochemistry are still held mainly at the organ and tissue level without the use of experiments with cell cultures (Muller, 2007). Using for such purposes the culture of cells in a complex picture of the ontogenetic changes helps to isolate the effect of genetic factors. Gene expression of several proteins with different mechanism of action and the quantity of their products has been investigated (metallothioneins 1– 5 types, ubiquitin, p53) All these proteins are protective ones, but perform their function using different mechanisms
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