Comparison of four serological tests for the diagnosis of swine brucellosis

Luis Antonio Mathias,Carolina De Alvarenga Cruz,Daniel Bartoli De Sousa,Raphaella Barbosa Meirelles-Bartoli,Reiner Silveira De Moraes,Eric Matheus Nascimento De Paula,Rafaela Assis Oliveira,Nivaldo Aparecido De Assis

doi:10.33448/rsd-v9i7.4418

Luis Antonio Mathias, Carolina De Alvarenga Cruz + Show 6 more

Open Access

https://doi.org/10.33448/rsd-v9i7.4418

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Abstract

The purpose of this article is to present results of a research in which the results of four serological tests were compared for the diagnosis of swine brucellosis in pigs from Brazilian herds in which the outbreak occurred in relation to other brucellosis-free. Rose Bengal (RBT), complement fixation (CFT), agglutination plus 2-mercaptoethanol test (TAT+ME), and commercial fluorescence polarization assay (FPA) were used to test 333 sera (271 sows and 62 finishing pigs) from a Brucella suis infected swine herd and 1,100 swine sera from brucellosis free pigs taken at a slaughterhouse. Considering infected all the 271 sows from the outbreak, and interpreting results of the FPA according to the manufacturer directions, sensitivities observed were 95.94% for FPA, 94.83% for RBT, 93.73% for CFT, and 92.25% for TAT+ME. Considering infected all the pigs from the infected herd with a positive result in at least one test, sensitivities observed were 98.92% for RBT, 97.13% for CFT, 96.06% for FPA, and 94.98% for TAT+ME. Specificities of the tests were CFT and TAT+ME, 100%; FPA, 99.55%; and RBT 99.27%. Results indicated a good performance of all the tests, and the agreement among them was almost perfect.

Highlights

Brucellosis is one of the most important infectious diseases of swine due to economic losses, restriction to international trade and risk of human infection
Changes in swine management practices and sanitary programs contributed to the reduction of swine brucellosis prevalence rates, but occasional outbreaks still occur in Brazil (Meirelles-Bartoli et al, 2012)
Different results were obtained by Stoffregen et al (2007) who observed lower sensitivities by using card test, which is similar to Rose Bengal test, standard tube test and fluorescence polarization assay, to diagnosis brucellosis in feral swine, enzootically infected and captured in the United States of America

Summary

Introduction

Brucellosis is one of the most important infectious diseases of swine due to economic losses, restriction to international trade and risk of human infection. Changes in swine management practices and sanitary programs contributed to the reduction of swine brucellosis prevalence rates, but occasional outbreaks still occur in Brazil (Meirelles-Bartoli et al, 2012). Research is carried out with the purpose of bringing new knowledge to society as stated by Pereira et al (2018). In the present study of a quantitative nature, serorological research was carried out.

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