Abstract
This study compared the extraction efficiency of 3 different techniques, ultrasound-assisted extraction (UAE), microwaveassisted extraction (MAE), and the classical extraction method, in obtaining some bioactive and allergen compounds, namely liquiritin (LQ), glycyrrhizin (GL), glycyrrhetinic acid (GA), and carbenoxolone (CBX), from liquorice samples of Glycyrrhiza glabra grown in Turkey. Extraction parameters for each technique were optimised by the response surface methodology-central composite design approach. Concentrations of LQ, GL, GA, and CBX, which were selected as the responses of selected independent variables, were determined by liquid chromatography-electrospray ionisation tandem mass spectrometry. One-way variance analysis was performed to evaluate the significance of the results. MAE was revealed as the most effective technique for extracting LQ (mean: 8.52 mg g-1), GL (mean: 76.65 mg g-1), and GA (mean: 1138.84 μg g-1) from liquorice, while the classical technique was the most effective for obtaining CBX (mean: 5.75 μg g-1).
Highlights
Liquorice (Glycyrrhiza) species belong to the legume family (Fabaceae) and are grown in Europe, Asia, North and South America, and Australia
The current study aims to demonstrate the effects of 3 different extraction methods, namely ultrasound-assisted extraction (UAE), microwaveassisted extraction (MAE), and classical extraction, on some bioactive and allergen compounds (GL, glycyrrhetinic acid (GA), LQ, and CBX) from liquorice samples of G. glabra grown in Turkey
This study compared the extraction efficiency of 3 different techniques, including UAE, MAE, and classical extraction, to extract some bioactive and allergen compounds (GL GA, LQ, and CBX) from liquorice samples of G. glabra grown in Turkey
Summary
Liquorice (Glycyrrhiza) species belong to the legume family (Fabaceae) and are grown in Europe, Asia, North and South America, and Australia. The current study aims to demonstrate the effects of 3 different extraction methods, namely UAE, MAE, and classical extraction, on some bioactive and allergen compounds (GL, GA, LQ, and CBX) from liquorice samples of G. glabra grown in Turkey. Technical parameters such as the temperature, time, solvent-to-raw material ratio, amplitude (%), and microwave power were optimised using RSM
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