Abstract
Objective To simultaneously determine the quantify liquiritin, glycyrrhetinic acid and glycyrrhizic acid in Xianlong-Jiedu Yin by high performance capillary electrophoresis (HPCE). Methods HPCE method was performed on a fused silica capillary coates 75 μm×85 cm (effective column length 78.5 cm) using 50 mmol/L borax solution-acetonitrile (95:5), with adjusted pH=8.50 as running buffer, lytical 22 kV, and electrokinetic injection 10 s, detected wave length of 254 nm, and temprature maintaining 25 ℃. Results The liquiritin, glycyrrhetinic acid and glycyrrhizic acid were completely separated within 30 minutes, showing good linear relationship in the range of 12-120, 15-150, 8-160 μg/ml (r>0.999 3). The average recoveries were 100.70%, 98.51%, 98.40% with RSD 2.49%, 2.79%, 0.40%, respectively. Conclusions The HPCE was suitable for the determination of liquiritin, glycyrrhetinic acid and glycyrrhizic acid in XianLong-JieDu Yin, which can be used for the quality control. Key words: Capillary electrochromatography; Liquiritin; Glycyrrhizic acid; Glycyrrhetinic acid; Xianlong-Jiedu Yin
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