Comparison of Erythrocyte Membrane Lipid Profiles between NAFLD Patients with or without Hyperlipidemia.

Wenbin Chen,Tian Guo,Jie Han,Ling Gao,Chunxiao Yu,Shanshan Shao,Tao Bo,Guowang Xu,Chunxiu Hu,Hu Cai,Zhenyu Yao,Qunye Zhang,Meng Zhao,Yilin Fu,Jiajun Zhao

doi:10.1155/2020/9501826

Abstract

Objectives. Nonalcoholic fatty liver disease (NAFLD) and hyperlipidemia (HL) are common metabolic disorders due to overnutrition and obesity. NAFLD is often associated with hyperlipidemia. The aim of this study was to identify and compare the erythrocyte membrane lipids profile in NAFLD patients with or without HL. Methods. A total of 112 subjects (with similar age and body mass index) were divided into four groups: (1) normal controls, (2) NAFLD alone, (3) HL alone, and (4) NAFLD combined with HL (NAFLD + HL). Lipid was extracted from the erythrocyte membrane, and lipid profiles of subjects were analyzed by liquid chromatography mass spectrometry (LC-MS). Results. Data sets from 103 subjects were adopted for lipidomic analysis. Significant changes of lipid species were observed in patient groups, especially in the HL group and NAFLD + HL group. The HL group showed increased level of most lipid species, and decreased level of most lipid species was observed in the NAFLD + HL group. The weight percent of myristic acid, stearic acid, erucic acid, and docosahexaenoic acid also showed distinct variation between different groups. Conclusions. NAFLD, HL, and NAFLD + HL all had an impact on lipid profiling of the erythrocyte membrane. The influence of NAFLD alone is less important compared with HL. Some lipids should be highlighted because of their specific role in cell function and systemic metabolism.

Highlights

Nonalcoholic fatty liver disease (NAFLD) is the nonalcoholinduced deposit of extra fat in hepatocytes, resulting in diseases ranging from hepatic steatosis (NAFL), steatohepatitis (NASH), fibrosis, and eventual cirrhosis and cancer.e prevalence of NAFLD worldwide ranges from 6.3% to 33% in the general population [1]
We investigated the alterations of erythrocyte membrane lipid profiling of grouped subjects with NAFLD, hyperlipidemia, or NAFLD accompanied with hyperlipidemia using an liquid chromatography mass spectrometry (LC-MS) based lipidomics approach
Minor lipid profile changes were observed between the NAFLD group and control group. e erythrocyte membrane of the HL group exhibited the highest levels of most lipid species

Summary

Introduction

Nonalcoholic fatty liver disease (NAFLD) is the nonalcoholinduced deposit of extra fat in hepatocytes, resulting in diseases ranging from hepatic steatosis (NAFL), steatohepatitis (NASH), fibrosis, and eventual cirrhosis and cancer.e prevalence of NAFLD worldwide ranges from 6.3% to 33% in the general population [1]. Nonalcoholic fatty liver disease (NAFLD) is the nonalcoholinduced deposit of extra fat in hepatocytes, resulting in diseases ranging from hepatic steatosis (NAFL), steatohepatitis (NASH), fibrosis, and eventual cirrhosis and cancer. NAFLD have an increased risk of developing type 2 diabetes and cardiovascular problems [2,3,4]. Increased liver load with lipids such as free fatty acids (FFA) [5] was shown to contribute to altered hepatic metabolism. Cholesterol, and low-density lipoproteins (LDL), combined with decreased high-density lipoproteins (HDL), represent acceleration in cardiovascular disease incidence. E presence of hyperlipidemia (HL) was reported in 20% to 80% cases associated with NAFLD [4]. NAFLD may impair hepatic lipid metabolism and initiate hyperlipidemia [6]

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Conclusion