Abstract
In this study a novel method was implemented and investigated in order to destroy cancer cells inside the mouse body on a clinical level. In the case of in-vitro study, MTT assay was employed to discover an effective dose of applied plasma and distinguish the plasma effect in direct and in indirect treatments. Tumor growth was also measured in in-vivo section so that the effectiveness of direct and indirect treatments could be compared. Furthermore, an investigation was conducted to study the interferences between a conventional method (chemotherapy) and plasma treatment so as to increase the effectiveness of treatment inside the body. Hematoxylin and Eosin, Flow Cytometry, TUNEL and Western Blot assay were used to investigate any cell alteration and the impact of various treatment methods on cancer cell and amount of their apoptosis and protein levels. Radiology and CT scan images were taken to determine the final tumor volume. The results showed a significant cell death and substantial reduction in tumor growth in direct plasma treatment in comparison with indirect plasma treatment. Eventually, dramatic destruction of cancer cells was observed while using of indirect plasma-chemotherapy combination, thus introducing an effective method for deep tissue tumors can be introduced.
Highlights
Plasma medicine has had a significant growth in recent years[1,2,3]
In order to show that this device has no thermal effects, cell cultured medium temperature was measured with Infra-Red thermometer after 6 minutes of plasma treatment and 1 ± 0.1 °c temperature increase was observed
The results showed that the number of cells in the treatment groups are less than the control group (Fig. 7), and there were no signs of burning in the observed tissues in treatment groups
Summary
Plasma medicine has had a significant growth in recent years[1,2,3]. Technologies such as plasma surgery to remove lesions was fundamentally based on plasma deadly effects on living systems[4,5]. Reaction of CAP with cancer cells in in-vivo and in-vitro shows anti-tumor effects[28,29,30,31,32,33] Such reaction is resulted from combination of physical and chemical factors. Utsumi et al showed that plasma activated medium reduced tumor size when injected into the mouse body They studied the effect of plasma activated medium on Ovary cancer cells both in-vitro and in-vivo. They observed that Glioblastoma cells were perished selectively by this medium They showed that extracellular signal-regulated kinase (ERK) and protein kinase B (AKT) signaling pathways were set in a low level by means of the plasma activated medium. That plasma to be used inside the body, chemotherapy and indirect plasma were combined
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