Abstract
Qualitative and quantitative determination of carotenoids pigments can provide valuable information about the organisms in which this important class of compounds is found. In the HPLC analysis of pigments, diode array (DAD), electrochemical (ED) and other kinds of detector may be used. The aim of this work is to develop an HPLC method using a C30 column to identify and quantify sixteen different pigments from algae. A further aim is to compare precision and accuracy obtained by DAD and ED. ED is normally more sensible than DAD. On the other hand, the highest precision and accuracy was obtained with DAD. In conclusion, the method was efficient for quantitative and qualitative analyses of pigments from cyanobacteria and different microalgae classes. Their pigment patterns for several organisms are also discussed.
Highlights
Carotenoids are pigments found mostly in plants and algae
Since carotenoids can be analyzed by either an electrochemical or a diode array detector, the aim of this work was to develop an HPLC method to identify and quantify sixteen carotenoids and chlorophylls from algae using a C30 column and to compare the precision and accuracy values and overall performance obtained with DAD and electrochemical detector (ED)
Many methods are available in the literature for analysis and quantification of carotenoids and chlorophylls in different matrices due to the importance and applicability of these compounds.[14,15,16,22,23,24]
Summary
Carotenoids are pigments found mostly in plants and algae. Their biological function in these organisms is to act as antioxidants,[1,2] membrane stabilizers[3,4] and light harvesters in photosynthetic organisms.[5]. Since carotenoids can be analyzed by either an electrochemical or a diode array detector, the aim of this work was to develop an HPLC method to identify and quantify sixteen carotenoids and chlorophylls from algae using a C30 column and to compare the precision and accuracy values and overall performance obtained with DAD and ED. Analyzed by varying the applied potential in each run.[28] With these data, it was possible to obtain hydrodynamic voltammograms, as shown in Figure 2 for b-carotene, lutein and chlorophyll a.
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