Abstract
Cu 2+, Ni 2+, Zn 2+, Co 2+ and Cd 2+ were evaluated in metal ion affinity chromatography for enrichment of selenoprotein P, and immobilized Co 2+ affinity chromatography was found to be the most selective chromatographic method. The chromatography was performed by fast protein liquid chromatography and the fractionation was followed by analysis of the collected fractions for selenium by inductively coupled plasma mass spectrometry. By the combination of immobilized Co 2+ affinity chromatography and heparin affinity chromatography a simple method was developed yielding a 14 800-fold enrichment of selenoprotein P. The purity of the protein was determined by SDS–PAGE and by sequencing from polyvinylidene diflouride blots of SDS–PAGE gels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
