Comparison of Deoxyribose Nucleic Acid Purification Methods of Mangosteen (Garcinia mangostana L.) and Its Relatives

Sulassih Sulassih,Edi Santosa

doi:10.32663/ba.v1i2.1469

Abstract

Mangosteen (Garcinia mangostana L.) and its relatives (Garcinia hombroniana, Garcinia celebica, Garcinia forbesii, Garcinia malaccensis, Garcinia porecta, Garcinia subeliptica, Chalophylum inophylum) contain polyphenol compound. The polyphenol compound makes pure deoxyribose nucleic acid is difficult to reveal. The aim of this research was to find the deoxyribose nucleic acid purification method of mangosteen leaves and its relatives. The research was conducted from January to August 2015 at the Center of Horticultural Tropical Studies Laboratory, Bogor Agricultural University. The mangosteen leaves were isolated based on cetyl trimethyl ammonium bromide (CTAB) buffer extraction added by 2X chloroform isoamyl alcohol (CIAA 24:1), 3X CIAA 24:1, and sliced gel purification using Fermentas kit extraction. The result showed that CTAB added by 2X CIAA was the best treatment for Garcinia mangostana L. and its relatives for purification of deoxyribose nucleic acid. This modified method produced an apparent amplified polymerase chain reaction using PKBT7 inter simple sequence repeat marker. It was applicable to evaluate genetic diversity interspecies.

Highlights

The deoxyribose nucleic acid (DNA)molecular methods have been known as the tool to determined interspecies identification (Zietkiewicz et al, 1994), genetic variability (Mohamad et al, 2017, Ahmad et al, 2010; Sangani, 2017; Daryono et al, 2019), transgenic analysis (Maaty & Oraby, 2019), mapping gene (Amom & Nongdam, 2017), and yield characters’ selection (Roberdi et al, 2015)
The DNA molecular methods based on polymerase chain reaction (PCR) need high-quality
Plant materials The sample leaves of Garcinia mangostana, Garcinia hombroniana, Garcinia porecta, Garcinia celebica, Garcinia forbesii, Calophyllum inophylum, Garcinia subelliptica, and Garcinia malaccensis were collected from the Center of Tropical Horticultural Studies, Mekarsari Fruit Garden, Bogor Botanical Garden, and the farmer fields (Table 1)

Summary

Introduction

Molecular methods have been known as the tool to determined interspecies identification (Zietkiewicz et al, 1994), genetic variability (Mohamad et al, 2017, Ahmad et al, 2010; Sangani, 2017; Daryono et al, 2019), transgenic analysis (Maaty & Oraby, 2019), mapping gene (Amom & Nongdam, 2017), and yield characters’ selection (Roberdi et al, 2015). The DNA molecular methods based on polymerase chain reaction (PCR) need high-quality. Optic density (OD) ratio (A260/A280) has been standardized to measure the purity of DNA at 1.8-2.0 (Becker et al, 1996). Garcinia mangostana L. is well known as a queen of fruit. Garcinia mangostana L. belonging to Guttiferae family contains yellow latex in the stem, leaves, flowers, and fruits. Garcinia mangostana L. and its relatives' leaves contain phenolic compounds

Objectives

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Results

Conclusion