Abstract
ABSTRACTAim: The aim of this study is to assess and compare the efficacy of Jordanian propolis and full concentration mature coconut water in their ability to preserve periodontal ligament (PDL) cell viability after exposure of PDL cells to up to 120 minutes dry storage.Materials and methods: PDL cells were obtained from sound permanent first molars which were cultured in Dulbecco's Modified Eagles Medium (DMEM). Cultures were subjected to 0, 30, 45, 60, 90 and 120 minutes dry storage times then incubated with 100% mature coconut water, Jordanian propolis and DMEM for 45 minutes at room temperature (18-26°C). Untreated cells served as controls at each dry storage time tested. PDL cell viability was assessed by MTT assay. Statistical analysis of data was accomplished by using one-way analysis of variance complemented by Tukey test and the level of significance was 5% ( p < 0.05).Results: Up to 60 minutes dry storage, no significant improvement on the percentage of viable cells was found from soaking in all tested media. On the other hand, soaking in mature coconut water only resulted in higher percentages of viable cells at >60 minutes dry storage. However, this improvement was not significant (p > 0.05).Conclusion: Avulsed teeth which have been left dry for <45 minutes should be replanted immediately, whereas avulsed teeth which have been left dry for >45 minutes may benefit from soaking for 45 minutes in mature coconut water.How to cite this article: Al-Haj Ali SN, Al-Jundi S, Mhaidat N. Comparison of Coconut Water and Jordanian Propolis on Survival of Bench-dried Periodontal Ligament Cells: An in vitro Cell Culture Study. Int J Clin Pediatr Dent 2013;6(3):161-165.
Highlights
Avulsion is one of the most severe traumatic injuries characterized by complete displacement of the tooth outside the socket.[1]
On the other hand, soaking in mature coconut water only resulted in higher percentages of viable cells at 60 minutes dry storage
There was a significant drop in the percentage of viable cells for untreated control cells, mature coconut water and PG propolis solution as the dry storage time progressed from 0 to 90 minutes ( p < 0.05), whereas the drop was insignificant from 90 to 120 minutes
Summary
Avulsion is one of the most severe traumatic injuries characterized by complete displacement of the tooth outside the socket.[1] Avulsion management focuses on replantation at the accident site.[2,3] this is rare to occur, as literature reported that replantation occurs most commonly between 1 and 4 hours after avulsion.[4] there are certain factors that should be considered prior to replantation including the extra-alveolar duration and the storage conditions.[5,6] These factors are directly related to the favorable conditions which include preservation of PDL cells’ viability and cementum integrity.[4]. Dry storage causes death of PDL cells.[7] many literature reports correlated dry storage with an increased incidence of ankylosis and replacement resorption.[8,9,10] Recently, coconut water and propolis were suggested as storage media for avulsed teeth.[2,3,11,12,13]
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