Abstract
Electrospinning technique is able to create nanofibers with specific orientation. Poly(vinyl alcohol) (PVA) have good mechanical stability but poor cell adhesion property due to the low affinity of protein. In this paper, extracellular matrix, gelatin is incorporated into PVA solution to form electrospun PVA-gelatin nanofibers membrane. Both randomly oriented and aligned nanofibers are used to investigate the topography-induced behavior of fibroblasts. Surface morphology of the fibers is studied by optical microscopy and scanning electron microscopy (SEM) coupled with image analysis. Functional group composition in PVA or PVA-gelatin is investigated by Fourier Transform Infrared (FTIR). The morphological changes, surface coverage, viability and proliferation of fibroblasts influenced by PVA and PVA-gelatin nanofibers with randomly orientated or aligned configuration are systematically compared. Fibroblasts growing on PVA-gelatin fibers show significantly larger projected areas as compared with those cultivated on PVA fibers which p-value is smaller than 0.005. Cells on PVA-gelatin aligned fibers stretch out extensively and their intracellular stress fiber pull nucleus to deform. Results suggest that instead of the anisotropic topology within the scaffold trigger the preferential orientation of cells, the adhesion of cell membrane to gelatin have substantial influence on cellular behavior.
Highlights
The behavior of cells, including cell adhesion, morphology, proliferation, and differentiation, is affected by surface topography
Since Poly(vinyl alcohol) (PVA) may be dissolved instantly in water, as-prepared nanofibers were cross-linked by glutaraldehyde vapor for 48 h soaked in a medium
Similar bands appear in 2935/2905 cm−1, representing CH2 asymmetric and symmetric stretching; 1605−1 cm is the vibration of C-O, and 1426 cm−1 is CH2 bending
Summary
The behavior of cells, including cell adhesion, morphology, proliferation, and differentiation, is affected by surface topography. Gelatin is a natural polymer similar to a kind of ECM, a derivation of collagen that is abundant in the skin, tendons, cartilage, and connective tissues of animals. It has been extensively used for wound dressings[7], drug delivery systems[8], or nerves[9]. Linh et al fabricated PVA-gelatin electrospinning nanofiber in water-acetic acid and deionized water solvent[15]. They studied the physical properties of nanofibers, and select aqueous solutions for dispersion to reduce the cytotoxicity of as-prepared nanofibers and improve the mechanical stability of nanofiber by gelatin. The morphological changes, surface coverage, viability and proliferation of cells growing on randomly oriented or aligned nanofibers
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