電紡 collagen/chitosan/PVA 奈米纖維支架為傷口敷料之研究

Abstract

Tissue engineering includes three major elements: cells, scaffolds, and signal molecules. We are focuses on how to prepare the mimic extracellular matrices whose components and porous structures are analogous to those of the native extracellular matrices (ECMs). Electrospun nanofibers have amazing characteristics such as very large surface area-to-volume ratio and high porosity with very small pore size. The process was an efficient technique for the fabricationof both natural and synthetic polymers nanofibers and various polymer have been electrospun. Collagen, chitosan, polyvinyl alcohol(PVA) are biodegradable, biocompatible materials. We using concentrated acetic acid solution(90%) as a solvent. Because more concentrated acetic acid in water progressively decreased surface tension of the chitosan solution and concomitantly increased charge density of jet. Electrospun collagen/chitosan/PVA nanofibers were crosslinked by glutaraldehyde(GTA) vapor to enhanced stability. The morphology of the electrospun collagen/chitosan/PVA nanofiber scaffolds was observed by scanning electron microscopy (SEM) and stabilized by glutaraldehyde (GTA) vapor via crosslinking. Fourier transform infrared spectra analysis showed that the collagen/chitosan/PVA nanofibers scaffold do not change significantly, except for enhanced stability after crosslinking by GTA vapor. The thermal behavior was studied by thermal gravimetric analysis and the mechanical properties were Studied by tensile testing. To assay the biocompatibility of electrospun scaffolds, cellular behavior on the nanofibrous scaffolds was also investigated by SEM, methylthiazol tetrazolium testing(MTT) and dsDNA content. The cyototoxic effecy of GA-vapor crosslinked scaffolds was observed. The scaffolds show almosted no cellular activity.