Abstract

1. 1. Fatty acids from the several lipid classes, previously separated by thin layer chromatography, were analyzed by gas-liquid chromatography in tissues from parr and mature coho salmon ( Oncorhynchus kisutch). 2. 2. The polyunsaturated fatty acid (PUFA) composition of the parr was markedly different from that of mature fish; the former being characterized by significantly higher amounts of w-6 PUFA in the plasma free fatty acid (FFA), plasma phospholipids (PL), muscle triglyceride (TG) and phospholipids. Mature fish and higher levels of w-3 long chain (PUFA) in FFA and TG lipid classes in both plasma and muscle. 3. 3. The ratio of C20:4 w-6/C20:5 w-3 in TG and PL of plasma and muscle was higher in parr than in mature fish, while the ratio in FFA was lowest in parr. 4. 4. The incorporation ratios of C20:4 w-6 into PL of muscle was much higher than that of C20:5 w-3 in both parr and mature fish, even though the content of C20:5 w-3 was more than C20:4 w-6 in fish tissue.

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