Comparison of Antioxidant Activity of Chitosan Extracted Via Different Extraction Methods

Abstract

Chitosan is a natural polysaccharide that has antioxidant properties. It can consider as an antioxidant agent in food supplements. In this study the effect of extraction methods on the antioxidant activity of chitosan was investigated. Three methods including enzymatic, acid and alkaline methods were applied for isolating the chitosan from shrimp waste. The content of extracted chitosan was measured and its antioxidant activity was investigated by employing various analyses such as assay of their power on the oxidative stability in sunflower oil and DPPH. Inhibition of lipid per-oxidation was evaluated by thiobarbituric acid reactive substances assay. The extracted chitosan by enzymatic method had best antioxidant activities. Furthermore, the results demonstrated that good yields can be obtained via enzymatic treatment. According to the results, the content of chitosan influences on antioxidant activity. The chitosan extracted by enzymatic method can be a source of antioxidant agents in manufacturing food and supplement.