Comparison of Antibacterial Activity of Ethanolic Fractions Separated from Trichoderma with the Commercial Antibiotic Methicillin against Drug-Resistant Bacteria using Minimum Inhibitory Concentration Method

Abstract

Aim: To compare the antibacterial activity of innovative ethanolic fractions of Trichoderma viride against Drug-resistant bacteria. Materials and Method: Trichoderma was isolated from the soil sample and the ethanolic extract was prepared from the fully grown mycelia. The ethanolic extract was tested against the four Methicillin-Resistant Staphylococcus aureus strains (MRSA). Clinicalc.com was used to calculate the sample size and found that alpha error-threshold at 0.05, 95% confidence interval, g power at 80% and the enrolment ratio to be 1. Sample size of each group was 12. Results: The MIC was determined to be Escherichia coli, Staphylococcus aureus and Bacillus subtilis-50 μg/mL and Pseudomonas aeruginosa-100 μg/mL and MRSA strains (all four) – 100 μg/mL. The statistical Independent Sample T-Test analysis showed significant antibacterial activity against MRSA for the Trichoderma ethanolic extract with the statistical significance (p<0.05) when compared to the Susceptible bacterial strains. Conclusion: The tested ethanolic extract inhibited the MRSA at 100 μg/mL.