Comparison of an Elisa and a Radial Immuno Diffusion (RID) Method for the Determination of Factor VIIIR: Ag

Abstract

This chapter presents a study in which a new ELISA and a relatively settled radial immuno diffusion (RID) determination method for the quantification of FVIIIR: Ag in human citrated plasma are evaluated. A number of advantages of the ELISA method over the RID method are discussed. The range of the ELISA method is broader (150% n to 1% n), whereas the RID method is limited from 150% n to about 30% n. The results of the FVIIIR: Ag ELISA determination are available within 4 hours, and those of the RID method are available hardly after one week. As a disadvantage, the ELISA technique is relatively cumbersome and in the practical performance relatively laborious. Moreover, the test kit is rather expensive, especially if small numbers of samples are to be done. Nevertheless, the ELISA method represents an interesting new tool for the determination of parameters in the field of clinical chemistry. The sensitivity and the analytical characteristics of the method introduced are better than the RID method, and the time necessary for the delivery of the results, but some experience with enzyme immuno assay is needed for a clean practical performance.