Abstract
ABSTRACT In the present study, a simple rapid test based on lactose fermentation, suggested in 1990 by Shrestha and Sinha for the rapid detection of coliforms in pasteurized milk, was evaluated. In the rapid test, a concentrate of the product (heat‐treated and inoculated with different levels of Escherichia coli – 102, 104and 106cells/mL), obtained by centrifugation, was mixed with an equivalent amount of double‐strength broth before incubation at 37C. A total of 60 samples of pasteurized milk was studied. Thirty of them were analyzed by the Most Probable Number (MPN) method and by the rapid test. Mac Conkey, Brilliant Green Bile (BGB) and Violet Red Bile (VRB) were used as selective broths. A statistically significant negative relationship between the MPN method and the rapid detection time (obtained by rapid test) was found. The other 30 samples were used to compare the MPN, the rapid test and the Petrifilm® methods for rapid detection of coliforms. The detection times using VRB were shorter. The differences among the three methods were statistically significant.
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