Comparison in antioxidant and antitumor activities of pine polyphenols and its seven biotransformation extracts by fungi.

Hui Li,Zhenyu Wang

doi:10.7717/peerj.3264

Abstract

Microbial transformation can strengthen the antioxidant and antitumor activities of polyphenols. Polyphenols contents, antioxidant and antitumor activities of pine polyphenols and its biotransformation extracts by Aspergillus niger, Aspergillus oryzae, Aspergillus carbonarius, Aspergillus candidus, Trichodermas viride, Mucor wutungkiao and Rhizopus sp were studied. Significant differences were noted in antioxidant and antitumor activities. The highest antioxidant activities in Trolox equivalent antioxidant capacity (TEAC), DPPH radical scavenging activity, superoxide anion radical scavenging activity, hydroxyl radical scavenging activity, reducing power assay and antitumor activity against LoVo cells were biotransformation extract of Aspergillus carbonarius (BAC), biotransformation extract of Mucor wutungkiao (BMW), biotransformation extract of Aspergillus carbonarius (BAC), biotransformation extract of Aspergillus niger (BAN), biotransformation extract of Aspergillus oryzae (BAO) and BMW, respectively. Correlation analysis found that antioxidant and antitumor activities were associated with polyphenols contents and types of free radicals and tumors. A. carbonarius can make polyphenol oxidation, hydroxylation and methylation, and form new polyphenols. In conclusion, A. carbonarius, A. niger and M. wutungkiao are valuable microorganisms used for polyphenols biotransformation and enhance the antioxidant and antitumor activities of polyphenols.

Highlights

The main ingredient of pine barks extracts is proanthocyanidins, followed by catechin-based flavonoids and phenolic acids
The aim of this study is to prove beneficial fungi in enhancing the polyphenols antioxidant and antitumor activities in vitro
Aspergillus niger, Aspergillus niveus and Aspergillus awamori fermented defatted soybean flour significantly increase in polyphenols content and fungi β-glucosidases might have hydrolysis β-glucoside linkages, mobilizing polyphenols to react with the Folin-Ciocalteau reagent (Georgetti et al, 2009)

Summary

Introduction

The main ingredient of pine barks extracts is proanthocyanidins, followed by catechin-based flavonoids and phenolic acids. These proanthocyanidins and catechin-based flavonoids have high potential values of physiological activities, such as antioxidants (Grimm, Schäfer & Högger, 2004), anti-tumors (Ma et al, 2008), anti-inflammatory (Peng et al, 2012), antineurodegenerative diseases (Pipingas et al, 2008), anti-atherosclerosis (Kim et al, 2010), anti-UV radiation (Kimura & Sumiyoshi, 2010) and anti-radiation (Li et al, 2016), etc. How to cite this article Li and Wang (2017), Comparison in antioxidant and antitumor activities of pine polyphenols and its seven biotransformation extracts by fungi. Aspergillus terreus can transform rutin into isoquercitrin with strong anti-inflammatory activity (Weignerova et al, 2012)

Objectives

Methods

Results

Conclusion