Abstract
The enzymatic, physicochemical and immunochemical properties of pig liver neutral α-glucosidase were compared with those of pig serum neutral α-glucosidase. The enzyme purified from pig′s liver showed a pH-optimum of 6.5 and was stable in the range of pH 5.0 to 9.0. This enzyme also showed a temperature-optimum of 60°C and was stable up to 60°C. These properties of pH and temperature of the pig liver α-glucosidase were very similar to those of the pig serum α-glucosidase reported previously. The isoelectric points and the molecular weights of the two α-glucosidases were same: pI, 4.2 and MW, 2.7˜3.0 × 105. The Km values for maltose, maltotriose and phenyl α-maltoside of both α-glucosidases were 8.5 mm, 2.2 mm and 2.3 mm, respectively. The essential ionizable groups of the enzymes were identified to be a carboxyl group (–COO−) in the acidic side and an imidazole group (?NH+) in the alkaline side. The pig liver α-glucosidase produced a clear precipitin line with the antiserum to the pig serum α-glucosidase,...
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