Comparison between the coagulase (coa and vwb) genes in Staphylococcus aureus and other staphylococci

Abstract

Out of 158 samples were collected from otitis media, burn wounds and throat of pneumonia patients, 53 Staphylococcus aureus were identified by 16S rDNA sequences, 50 isolates were coagulase positive, while 3 as coagulase negative. The coa gene encoding for Coa was amplified only in 41 (77.3%) of S. aureus isolates (including the three coagulase negative isolates) while the vwb was amplified in all S. aureus isolates. The phylogenetic relationships among coagulases genes of different species of Staphylococci revealed clear differences between coa gene from S. aureus isolate and the genes encoding coagulase from non-S. aureus species. S. aureus have two coagulase-encoding genes (coa and vwb genes), while the other species (Staphylococcus pseudintermedius, Staphylococcus schleiferi, Staphylococcus intermedius and Staphylococcus hyicus) have only one coagulase gene, which closer to the vwb than coa.The present study conducted to compare between the coagulase genes produced by Staphylococci sp.