Abstract
Bovine brucellosis is a zoonosis known to be a major public health hazard of great economic importance worldwide. Its detection in cattle is frequently done using serological tests like Rose Bengal Plate Test (RBPT) and Competitive- Enzyme Linked Immuno Sorbent Assay (C-ELISA). Few studies have been conducted to compare the serological tests most commonly used to detect bovine brucellosis in Rwanda. The study was conducted in Kigali city, the capital of Rwanda, to compare RBPT and C-ELISA test in detection of bovine brucellosis. Two thousands and seventeen (2017) cattle serum samples were collected, from 157 randomly selected farms, and were tested for bovine brucellosis using RBPT and C-ELISA. The overall prevalence given by RBPT (2.03%) was greater than the prevalence given by C-ELISA (1.7%). The study revealed that even if the observed difference between prevalence given by RBPT and the prevalence given by C-ELISA was significant (p-value<0.05), the agreement between the two tests has been found excellent with a Kappa of 0.92. The study has provided information on the agreement of the two serological tests commonly used to detect bovine brucellosis in Kigali. It is known that the agreement between two tests varies depending on the prevalence, thus there is a need for the study to be extended to the other provinces of Rwanda where the prevalence is probably different to the prevalence in Kigali.
Highlights
Bovine brucellosis is an infectious and contagious disease usually caused by Brucella abortus, less frequently by Brucella melitensis, and occasionally by Brucella suis [1].It is a major zoonosis with an important economic and public health impact
Presence of agglutination was regarded as positive.All sera were tested by the Competitive- Enzyme Linked Immuno Sorbent Assay (C-ELISA), SVANOVIR®Brucella-Ab C-ELISA (Svanova, Sweeden) using the protocol described by the manufacturer: In this procedure, the samples are exposed to Brucella abortus smooth lipopolysaccaride (S-LPS) coated wells on microtiter plates together with mouse monoclonal antibody specific for an epitope on the o-poly-saccharide portion of the S-LPS antigen
With Rose Bengal Plate Test (RBPT) an overall prevalence of 2.03% was obtained while C-ELISA test gave a prevalence of 1.7% .Only female were found to be infected
Summary
Bovine brucellosis is an infectious and contagious disease usually caused by Brucella abortus, less frequently by Brucella melitensis, and occasionally by Brucella suis [1]. Brucellosis in humans is characterized by a febrile flu-like syndrome, frequent chills, headaches and general weakness [3] In cattle, it is usually spread by the vaginal discharge of an infected cow or an aborted foetus. Rose Bengal plate test (RBPT) is based on agglutination of colored particulate antigen (killed Brucella organisms) by the antibodies present in the patient’s serum [6]. It is a simple, cheap and effective test, the RBPT is generally considered to be less sensitive than other tests like standard tube agglutination test (STAT), complement fixation test (CFT) and ELISA [6,7]. The present study was undertaken to compare two serological tests (RBPT and C-ELISA test) in detection of bovine brucellosis in Kigali city
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
