Abstract
Three methods of introducing virgin queens into mating hives were compared with the standard method of using a ripe queen cell. Virgins were introduced into queenless mating nucleus hives (5-frame medium Langstroth) using a wooden mailing cage with candy and attendant bees and were released three days later. When the caged virgins were 0-1 days old when introduced, the proportion giving rise to an egg-laying queen (65%) was almost as high as with cells (70%) (P = 0.63, test). Success dropped to 55% (P = 0.04) for 3-4 day old virgins. If the virgin was introduced into a queenright mating hive for three additional days before the colony queen was removed the success rate was only 29% (P = 0.001). Across all methods, most queen loss occurred in the introduction/emergence period, with only one third in the mating period. The time taken to start egg laying, which averaged 12.9 days from emergence, did not vary significantly among methods.
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