Comparative transcriptomic and proteomic analyses of the green and white parts of chimeric leaves in Ananas comosus var. bracteatus.

Yanbin Xue,Zhuo Huang,Lingxia Sun,Fuxing Jiang,Jun Ma,Zhen Lin,Fatima Rafique,Xi Li,Yujue Zhou,Sanmiao Yu,Yehua He,Yingyuan Xiong,Mingdong Ma

doi:10.7717/peerj.7261

Yanbin Xue, Zhuo Huang + Show 11 more

Open Access

https://doi.org/10.7717/peerj.7261

Copy DOI

Abstract

BackgroundAnanas comosus var. bracteatus has high ornamental value due to its chimeric leaves. However, the chimeric trait is very unstable in red pineapple plants, and transcriptional variation between the two types of cells (white/green cells) and the molecular mechanism responsible for their albino phenotype remain poorly understood.MethodsComparative transcriptomic and proteomic analyses of the white parts (Whs) and green parts (Grs) of chimeric leaves were performed.ResultsIn total, 1,685 differentially expressed genes (DEGs) (712 upregulated and 973 downregulated) and 1,813 differentially abundant proteins (DAPs) (1,018 with low abundance and 795 with high abundance) were identified. Based on Gene Ontology (Go) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, the DEGs were mostly involved in carbon fixation in photosynthetic organisms, porphyrin and chlorophyll metabolism and oxidative phosphorylation, while proteomic analysis revealed that DAPs were mostly related to ribosomes, photosynthesis, photosynthesis antennas, and porphyrin and chlorophyll metabolism. Combined analysis showed increased mRNA levels but low abundance of nine proteins level in Whs /Grs related to photosynthetic pigment and photosynthesis. Transcriptional changes, posttranscriptional regulation and translational alterations of key enzymes involved in chlorophyll biosynthesis and photosynthesis may play important roles in the albino parts of chimeric leaves.

Highlights

Ananas comosus var. bracteatus is an herbaceous perennial monocot that belongs to the Bromeliaceae family (Collins, 1968)
Chl a decreased significantly in white parts (Whs), accounting for only 3.21% of that in green parts (Grs), and Chl b in Whs accounted for 14.94% of that in Grs
Unigene sequences were queried via BLAST against the following databases: ncbi-nr database, the SwissProt database, the eggNOG database, the Gene Ontology (GO) database and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database

Summary

Introduction

Ananas comosus var. bracteatus is an herbaceous perennial monocot that belongs to the Bromeliaceae family (Collins, 1968). Bracteatus is an herbaceous perennial monocot that belongs to the Bromeliaceae family (Collins, 1968) Plants of this family are mostly cultivated commercially for their fruit, the high-quality silk fiber of their stem and their chimeric leaves. Bracteatus has high ornamental value due to its green/white chimeric leaves. Comparative transcriptomic and proteomic analyses of the green and white parts of chimeric leaves in Ananas comosus var. Leaf cross-sections have revealed that the chimeric leaves of this plant are composed of normal green cells and albino white cells. The chimeric trait is very unstable in red pineapple plants, and transcriptional variation between the two types of cells (white/green cells) and the molecular mechanism responsible for their albino phenotype remain poorly understood. Comparative transcriptomic and proteomic analyses of the white parts (Whs) and green parts (Grs) of chimeric leaves were performed. Transcriptional changes, posttranscriptional regulation and translational alterations of key enzymes involved in chlorophyll biosynthesis and photosynthesis may play important roles in the albino parts of chimeric leaves

Methods

Results

Conclusion