Abstract
Salvia splendens is a perennial, ornamental herbaceous flower that is widely cultivated as a bedding plant in gardens. The development of novel S. splendens cultivars and investigating the relevant molecular mechanisms are of great significance. In this study, RNA-sequencing and real-time PCR methods were used to analyze the possible molecular mechanism of S. splendens mutant, SX919M. From the wild-type S. splendens 919CK, we firstly selected a natural mutant, SX919M, which displayed multiple branches, clustered spheroids, and radial symmetrical inflorescence with higher numbers of calyces, ovules, stamens, and perianth tubes. Further, the RNA-seq was used to identify the differentially expressed genes (DEGs) in the mutant which included a total of 3568 upregulated and 3290 downregulated unigenes. We further observed that the indole alkaloid biosynthesis pathway showed the highest DEG enrichment, which was supported by a significant increase in the IAA content in mutant SX919M. In addition, we validated three DEGs, namely, CL2200.Contig2_All encoding methyl IAA esterase, CL12462.Contig1_All and CL12462.Contig2_All, which encoded strictosidine synthase, upregulated in mutant SX919M. We selected a novel S. splendens germplasm SX919M with a high ornamental value and determined that the upregulation of IAA biogenesis may be associated with its development.
Highlights
Salvia L., with nearly 1000 species of shrubs, herbaceous perennials, and annuals, is the largest genus in the mint family (Lamiaceae: Nepetoideae: Mentheae: Salviinae) [1,2,3,4]
S. splenden mutant SX919M is a natural mutant of the stable cultivar S. splenden Ker-Gawler Qiji, which was produced by the Beijing Institute of Landscape Architecture
When compared with the wild-type, SX919M is characterized with multiple branches, clustered spheroids, and radial symmetrical inflorescence (Fig 1)
Summary
Salvia L., with nearly 1000 species of shrubs, herbaceous perennials, and annuals, is the largest genus in the mint family (Lamiaceae: Nepetoideae: Mentheae: Salviinae) [1,2,3,4]. The genus is widely distributed throughout the world. Development of a Salvia splendens Ker-Gawler mutant, SX919M. The funders had no role in the study design, data collection analysis, decision to publish, or preparation of the manuscript
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