Comparative Transcriptome Analysis Identifying the Different Molecular Genetic Markers Related to Production Performance and Meat Quality in Longissimus Dorsi Tissues of MG × STH and STH Sheep.

Shuru Cheng,Xia Zhang,Yuqin He,Jinping Shi,Lei Yang,Quanwei Zhang,Xueying Wang

doi:10.3390/genes11020183

Abstract

Crossbred sheep have many prominent traits, such as excellent production performance and high-quality meat, when compared to local sheep breeds. However, the genetic molecular markers related to these characteristics remain unclear. The crossbred MG × STH (small-tailed Han sheep (STH) × Mongolian sheep (MG)) breed and the STH breed were selected to measure production performance and meat quality. We used 14 indexes of production performance and meat quality, which in the MG × STH population showed significant differences compared to the STH breed. Subsequently, the longissimus dorsi from the two sheep were subjected to comparative transcriptomic analyses to identify differentially expressed genes (DEGs) related to production performance and meat quality. A total of 874 DEGs were identified between the two sheep groups. A total of 110 unique DEGs related to sheep production performance and meat quality were selected as the candidate DEGs. We found 6 production-performance-related and 30 meat-quality-related DEGs through a correlation analysis, including SPARC, ACVRL1, FNDC5, and FREM1. The expression levels of 11 DEGs were validated by real-time PCR, and the results were in accordance with the results of the comparative transcriptomic and correlation analyses. These results will assist in understanding sheep heterosis and molecular marker-assisted selection.

Highlights

Mutton is an important protein source for humans and accounts for a large percentage of meat consumption in China [1]
These results will assist in understanding sheep heterosis and molecular marker-assisted selection
Sheep population was greater than that of the small-tailed Han (STH) sheep (Figure 1H). These results suggest that the Mongolian sheep (MG) sheep crossbred with the STH sheep had improved production performance, but the meat quality was inferior to that of STH sheep

Summary

Introduction

Mutton is an important protein source for humans and accounts for a large percentage of meat consumption in China [1]. With improvements in human living standards, the demand for high-quality meat and nutrition has increased. The production of mutton has steadily increased in China, but the increasing demand for high-quality meat mutton has not yet been satisfied [2]. Increasing the production performance and improving the meat quality of sheep breeds are pressing issues. Small-tailed Han sheep (STH) and Mongolian sheep (MG), two indigenous sheep breeds famous for their high-quality meat and strong adaptability, are farmed widely in northwestern. There are significant differences between the STH and MG sheep breeds in terms of

Objectives

Methods

Results

Discussion

Conclusion