Abstract
Foot rot caused by the oomycete Phytophthora capsici is the serious most disease in black pepper, leading to complete crop losses. Even though the cultivars field tolerant to this disease have been identified, molecular mechanisms for the tolerance remain unknown. This study had the objective to identify the candidate genes involved in the foot rot field tolerance of black pepper through comparative transcriptome analysis. P. capsici tolerant/moderately resistant cultivar IISR-Shakthi and susceptible cultivar IISR-Subhakara were artificially inoculated under controlled conditions and mRNAs were isolated from the leaves of treated and control plants at 0, 2, 4, 6, 12, 24, 48 h after inoculation. Transcriptome profiles were compared using DD-RT PCR on cDNA fragments and differentially expressed bands were sequenced. Sequence analysis revealed the involvement of signal proteins and defence enzymes Premnaspirodiene oxygenase, Phosphatase 2C-like domain-containing protein, Nitrous oxide reductase family maturation protein, putative disease resistance protein RGA3, Aspartyl protease, beta-glucosidase enzyme, Cytochrome P450 signal protein, Serine/Threonine Protein kinase WAG1 and nucleoredoxin 1-1 enzyme in field tolerance of black pepper to foot rot. Pathway analysis followed had paved additional insights in to the molecular mechanism of tolerance.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
