Comparative toxic effect of ammonia exposure on Mauremys sinensis and invasive species Trachemys scripta elegans

Abstract

As one of main pollutants, ammonia could cause adverse effects to aquatic animals. To explore the toxic effects of ammonia on Chinese striped-necked turtles (Mauremys sinensis) and invasive species red-eared slider (Trachemys scripta elegans), we compared the activities of antioxidant enzymes, the mRNA levels of genes involved in immune status, endoplasmic reticulum stress and apoptosis between T. s. elegans and M. sinensis under ammonia exposure for 30 days. The results showed that ammonia obviously increased the activities of SOD, CAT, GPX and T-AOC in both T. s. elegans and M. sinensis, especially CAT and GPX in T. s. elegans were higher than that in M. sinensis. The expression levels of JAK, RELA and Mcl-1 in T. s. elegans obviously increased, while IL-6 mRNA levels significantly increased in M. sinensis. In addition, Bip and IRE1 levels in M. sinensis showed a marked increase, and were significantly higher than that in T. s. elegans. Bcl-2 and Bcl-xL transcriptional levels in T. s. elegans showed an increase, especially Bcl-xL were significantly higher than that in M. sinensis. These results indicated that T. s. elegans exhibited more stronger antioxidant defense and immune function than M. sinensis under ammonia exposure. M. sinensis was more likely to occur endoplasmic reticulum stress and inflammation in ammonia environment. This research reveals the physiological response of turtles to ammonia, helps to understand adverse effects of environmental pressure on aquatic turtles, and further explains the tolerance of invasive species T. s. elegans to environmental pollution.