Comparative Study on Population Ecological Distribution and Extracellular Enzyme Activities of Endophytic Fungi in &amp;lt;i&amp;gt;Artemisia annua&amp;lt;/i&amp;gt;

Zhuangzhuang Liu,Zhen Zhang,Yanna Lv,Bo Lian,Hangao Li,Yan Chen,Yanyan Zhao,Zhihao Ji

doi:10.4236/jbm.2019.78008

Abstract

The endophytic fungi in different tissues of Artemisia annua was isolated and purified to explore their ecological distribution and tissue preference, and the extracellular enzyme activities of dominant endophytic fungi were determined to characterize the metabolic function of endophytic fungi. The results showed that a total of 67 endophytic fungi were obtained from Artemisia annua tissues. The number and species of endophytic fungi in different tissues were significantly different. The number, colonization rate (CR) and isolation rate (IR) of endophytic fungi in root were significantly higher than those of stem and leaf. The dominant endophytic fungi, diversity and similarity coefficient of endophytic fungi also showed significant difference among tissues. The extracellular enzyme activities of endophytic fungi in different tissues are significantly different. The enzyme activities of endophytic fungi isolated from root are significantly higher than those isolated from stem and leaf. The research results showed that the endophytic fungi in Artemisia annua had significant tissue preference, and the metabolic function of endophytic fungi showed significant difference among tissues. This will lay a foundation for further research, development and utilization of endophytic fungi, and also provide a theoretical basis for screening functional endophytic fungi in Artemisia annua.

Highlights

Artemisia annua is the traditional Chinese medicine, and it contains volatile oils, artemisinin, flavonoids as well as other various bioactive substances [1]
The enzyme activities of endophytic fungi isolated from root are significantly higher than those isolated from stem and leaf
The research results showed that the endophytic fungi in Artemisia annua had significant tissue preference, and the metabolic function of endophytic fungi showed significant difference among tissues

Summary

Introduction

Artemisia annua is the traditional Chinese medicine, and it contains volatile oils, artemisinin, flavonoids as well as other various bioactive substances [1]. It has strong adaptability, wide distribution and many effects such as relieving heat and fever [2], as the source of artemisinin for malaria treatment. Research has shown that endophytic fungi widely exist within plant tissues without causing apparent harm to the host [4]. They are rich in species diversity and co-evolve with the host. Endophytic fungi have important ecological significance in the process of plant succession

Methods

Results

Discussion

Conclusion